A FQHPSFI peptide selectively binds to LPS activated alveolar macrophages and inhibits LPS induced MIP-2 production

丁宁¹
广州市第一人民医院麻醉科

Objective: To identify peptides selectively binding to LPS-activated alveolar macrophages (LAMs) and characterize their effects on the production of LPS-induced cytokines.Methods: A phage display library was screened by binding phages to unmanipulated AMs and then to LAMs. Individual clones were identified by ELISA. Positive clones were characterized by sequencing. Binding specificity of the selected phage was tested using immunofluorescent staining. The selected peptide was synthesized to determine whether it could modulate LPS-induced cytokine production.Results: 22 out of 40 clones selected after 4 rounds of biopanning bound selectively to LAMs. A clone displaying FQHPSFI peptide bound effectively to LAMs. Furthermore, FQHPSFI peptide competitively inhibited the binding of the clone to LAMs. Importantly, FQHPSFI peptide significantly inhibited MIP-2 production. Conclusions: The identified FQHPSFI peptide may be used for the modulation of LPS-stimulated MIP-2 production in AMs.