Effect of Paeoniflorin on TLR2 mediated the inflammatory response signal transduction pathways
张倩1 周苏明1
江苏省人民医院老年ICU
Objective：To investigate the effects of paeoniflorin (Pae) on the TLR2 signaling pathway in human THP-1 cell line stimulated by BLP.
Methods: Pae at various concentrations incubated with BLP stimulated THP-1 cells. TNF-α and IL-6 were detected using available ELISA kit. Pae incubated with BLP stimulated THP-1 cells. Western blot was used to test the expression of NF-κB in nuclear and TLR2, MyD88 in cytoplasm. The impact of Pae and BLP on cell apoptosis of THP-1 cells was estimated by flow cytometry.
Results: Pae dose-dependently inhibited the levels of TNF-α and IL-6 that were increased by BLP stimulation. Treatment with BLP led to an activation of NF-κB p65 and this activation was inhibited by Pae, whereas no significant differences in TLR2 and MyD88 expression were observed. There was no influence on BLP- induced cell apoptosis by Pae in THP-1 cells.
Conclusion: Pae has the capacity to inactivate inflammatory response through inhibiting NF-κB activity of THP-1 cells.
